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Lonza
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Image Search Results
Journal: Stem Cell Research & Therapy
Article Title: Induced pluripotent stem cells derived from patients carrying mitochondrial mutations exhibit altered bioenergetics and aberrant differentiation potential
doi: 10.1186/s13287-023-03546-7
Figure Lengend Snippet: Human patient dermal fibroblasts reprogrammed into hiPSCs using mRNA–microRNA approach. a Reprogramming schematic for mRNA and microRNA transfections. b Bright-field images (10×) of human disease fibroblasts undergoing reprogramming. Day 0 fibroblasts displayed flat and elongated morphology typical of fibroblasts. Days 3 transfected cells show morphological changes including a rounded morphology and apoptosis, which are consistent with the reprogramming process. Days 10–15 cultures show colony formation, a key iPSC characteristic when cells are in contact with fibroblasts. Multipolar spindle-shaped fibroblast cells transitioning to a more compact cobblestone appearance at day 10, with early stem cell colonies arising by day 11–13 and expanding into well-circumscribed pluripotent stem cell colonies at day 15. Isolated stem cells maintain characteristic well-circumscribed morphology with minimal spontaneous differentiations through 20 + passages. iPSC, induced pluripotent stem cell; c Doubling time analyses indicate that all hiPSCs exhibit similar proliferation times representative of actively dividing cells
Article Snippet: Putative hiPSCs were generated using the StemRNA 3rd Generation Reprogramming Kit for Adult and
Techniques: Transfection, Isolation
Journal: Cell Proliferation
Article Title: Manufacturing clinical‐grade human induced pluripotent stem cell‐derived beta cells for diabetes treatment
doi: 10.1111/cpr.13232
Figure Lengend Snippet: Comparison of Sendai virus, episomal and mRNA reprogramming methods for clinical and commercial use
Article Snippet: Source companies with rights to reprogramming kits (non‐exhaustive list) , Thermo Fisher Scientific (CytoTune‐iPS Sendai Reprogramming Kit) , Thermo Fisher Scientific (Epi5 Episomal iPSC Reprogramming Kit)
Techniques: Comparison, Virus, Plasmid Preparation, Transfection
Journal: Cell Proliferation
Article Title: Manufacturing clinical‐grade human induced pluripotent stem cell‐derived beta cells for diabetes treatment
doi: 10.1111/cpr.13232
Figure Lengend Snippet: Summary of relevant in‐process and final product testing during hiPSC‐derived β‐cell manufacturing
Article Snippet: Source companies with rights to reprogramming kits (non‐exhaustive list) , Thermo Fisher Scientific (CytoTune‐iPS Sendai Reprogramming Kit) , Thermo Fisher Scientific (Epi5 Episomal iPSC Reprogramming Kit)
Techniques: Sterility, Enzyme-linked Immunosorbent Assay, Cell Counting, Staining, Flow Cytometry, Marker, Expressing, In Vivo, Ubiquitin Proteomics, Calcium Flux Assay, Functional Assay, Activity Assay, Sequencing, TRAP Assay
Journal: Cell Proliferation
Article Title: Manufacturing clinical‐grade human induced pluripotent stem cell‐derived beta cells for diabetes treatment
doi: 10.1111/cpr.13232
Figure Lengend Snippet: Technical considerations for the expansion and differentiation of human induced pluripotent stem cells. Technical considerations include the cell inoculation method, feeding strategies and choice of two‐dimensional (2D) or three‐dimensional (3D) culture vessel. In general, 3D culture systems have a wider range of cell inoculation methods and feeding strategies that are applicable, and are more scalable than the 2D static counterpart. This figure is created with BioRender.com
Article Snippet: Source companies with rights to reprogramming kits (non‐exhaustive list) , Thermo Fisher Scientific (CytoTune‐iPS Sendai Reprogramming Kit) , Thermo Fisher Scientific (Epi5 Episomal iPSC Reprogramming Kit)
Techniques: